The Dakewe 2030411 PHA standard is a lyophilized lectin powder for ELISPOT assays, reconstituted in sterile PBS and stored at -20 °C.
The PHA standard (Cat# 2030411) is a lyophilized lectin derived from Phaseolus vulgaris (red kidney bean). It binds to PHA receptors on T‑lymphocytes and induces a non‑specific proliferative response, making it suitable for ELISPOT experiments.
To use, reconstitute the powder with 500 µL sterile PBS, mix, and let stand a few minutes. The resulting solution has a concentration of 25 µg/mL; for a typical well add 10 µL to achieve a working concentration of 2.5 µg/mL (suitable for 10⁵ PBMC). Store the lyophilized product at –20 °C, avoid repeated freeze‑thaw cycles, and use within 12 months. The preparation is filtered through a 0.22 µm membrane and meets endotoxin limits for ELISPOT assays. This manual is written in Chinese.
| Source organism | Phaseolus vulgaris (red kidney bean) |
|---|---|
| Form | Lyophilized powder |
| Reconstitution volume | 500 µL sterile PBS |
| Reconstituted concentration | 25 µg/mL |
| Working concentration | 2.5 µg/mL per well |
| Filtration | 0.22 µm membrane |
| Endotoxin level | Meets ELISPOT requirements |
| Shelf life | 12 months |
Add 500 µL sterile PBS to the lyophilized powder, let stand a few minutes, then mix to obtain a 25 µg/mL solution.
Filter the solution through a 0.22 µm membrane; it does not adversely affect cell culture.
Yes, the endotoxin content meets the required range for ELISPOT experiments.