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- Manuals
- 2
- Languages
- ZH
- Last added
- 2026-09-29
Featured DAKEWE manuals
Dakewe DKW-ST-PIPMA PMA + Ionomycin Standard Product Manual
The DKW‑ST‑PIPMA standard is a lyophilized mixture of PMA (phorbol 12‑myristate 13‑acetate) and Ionomycin, commonly used as stimulants in in‑vitro cell culture assays such as ELISPOT. Each vial contains 5 ng of PMA and 2.5 µg of Ionomycin.
To prepare a working solution, reconstitute the powder with 500 µL sterile PBS, let it stand briefly, and mix thoroughly. Use 10 µL of this solution per ELISPOT well (100 µL cell culture) to stimulate approximately 1 × 10⁵ PBMCs. Store the sealed vials at –20 °C, protected from light, and discard after a single use; avoid repeated freeze‑thaw cycles. The product remains stable for 12 months under these conditions. This manual is written in Chinese.
Covers: Product Description · Usage Instructions · Safety Precautions · Storage and Shelf Life · Related Reagents
Dakewe 2030411 PHA Standard Product Manual
The PHA standard (Cat# 2030411) is a lyophilized lectin derived from Phaseolus vulgaris (red kidney bean). It binds to PHA receptors on T‑lymphocytes and induces a non‑specific proliferative response, making it suitable for ELISPOT experiments.
To use, reconstitute the powder with 500 µL sterile PBS, mix, and let stand a few minutes. The resulting solution has a concentration of 25 µg/mL; for a typical well add 10 µL to achieve a working concentration of 2.5 µg/mL (suitable for 10⁵ PBMC). Store the lyophilized product at –20 °C, avoid repeated freeze‑thaw cycles, and use within 12 months. The preparation is filtered through a 0.22 µm membrane and meets endotoxin limits for ELISPOT assays. This manual is written in Chinese.
Covers: Product Description · Reconstitution Procedure · Working Concentration and Application · Filtration and Endotoxin Specification · Storage Conditions and Shelf Life
Latest DAKEWE manuals
Dakewe DKW-ST-PIPMA PMA + Ionomycin Standard Product Manual
Product Leaflet 1 pages · 110 KB · ZHThe Dakewe DKW-ST-PIPMA manual describes the PMA + Ionomycin lyophilized standard, its composition, reconstitution, usage for ELISPOT, storage, and safety.
Dakewe 2030411 PHA Standard Product Manual
Product Leaflet 1 pages · 93 KB · ZHThe Dakewe 2030411 PHA standard is a lyophilized lectin powder for ELISPOT assays, reconstituted in sterile PBS and stored at -20 °C.
Questions answered in DAKEWE manuals
How should the PMA + Ionomycin standard be reconstituted?
Add 500 µL sterile PBS to the lyophilized powder, let it stand briefly, then mix thoroughly to obtain the working solution.
From: Dakewe DKW-ST-PIPMA PMA + Ionomycin Standard Product Manual
What is the recommended storage condition for the standard?
Store the sealed vials at –20 °C, protected from light, and use within 12 months.
From: Dakewe DKW-ST-PIPMA PMA + Ionomycin Standard Product Manual
How should the PHA standard be reconstituted?
Add 500 µL sterile PBS to the lyophilized powder, let stand a few minutes, then mix to obtain a 25 µg/mL solution.
What filtration step is required before using the reconstituted PHA solution?
Filter the solution through a 0.22 µm membrane; it does not adversely affect cell culture.