The Dakewe DKW-ST-PIPMA manual describes the PMA + Ionomycin lyophilized standard, its composition, reconstitution, usage for ELISPOT, storage, and safety.
The DKW‑ST‑PIPMA standard is a lyophilized mixture of PMA (phorbol 12‑myristate 13‑acetate) and Ionomycin, commonly used as stimulants in in‑vitro cell culture assays such as ELISPOT. Each vial contains 5 ng of PMA and 2.5 µg of Ionomycin.
To prepare a working solution, reconstitute the powder with 500 µL sterile PBS, let it stand briefly, and mix thoroughly. Use 10 µL of this solution per ELISPOT well (100 µL cell culture) to stimulate approximately 1 × 10⁵ PBMCs. Store the sealed vials at –20 °C, protected from light, and discard after a single use; avoid repeated freeze‑thaw cycles. The product remains stable for 12 months under these conditions. This manual is written in Chinese.
| PMA content per vial | 5 ng |
|---|---|
| Ionomycin content per vial | 2.5 µg |
| Reconstitution volume | 500 μL sterile PBS |
| Storage temperature | -20 °C, protected from light |
| Shelf life | 12 months |
Add 500 µL sterile PBS to the lyophilized powder, let it stand briefly, then mix thoroughly to obtain the working solution.
Store the sealed vials at –20 °C, protected from light, and use within 12 months.
Add 10 µL of the working solution to each well containing 100 µL cell culture, which works for about 1 × 10⁵ PBMCs.
No. Use the solution once and avoid repeated freeze‑thaw cycles.