The Dakewe DKW 33-R0100/DKW 33-R0400 mouse lymphocyte separation medium manual provides preparation, usage, and storage instructions for the…
This manual describes Dakewe’s mouse lymphocyte separation medium, a density‑gradient solution based on Iodixanol. It is used to isolate lymphocytes from mouse, rat, and rabbit spleens or peripheral blood mononuclear cells with high purity and yield.
The document details the step‑by‑step experimental procedure, including tissue grinding, centrifugation parameters (800 g, 30 min at room temperature), and post‑centrifugation washing. Storage conditions, safety notes, and troubleshooting tips are also provided. This manual is written in Chinese.
| Density | 1.0810 ± 0.0005 g/ml (20 °C) |
|---|---|
| Osmotic Pressure | 280 ± 15 mOsmol/kg |
| Endotoxin Level | <0.5 EU/ml |
| Package Size | 100 ml per bottle |
| Main Component | Iodixanol |
| Unopened Storage Temperature | 4‑30 °C |
| Opened Storage Temperature | 4‑8 °C (up to 2 weeks) |
Unopened bottles should be kept at 4‑30 °C; after opening, store at 4‑8 °C in sterile conditions and use within two weeks.
Centrifuge at 800 g for 30 minutes at room temperature, using a slow acceleration and deceleration (third gear if ten‑step).