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Dakewe DKW 33-R0100/DKW 33-R0400 Mouse Lymphocyte Separation Medium Manual
This manual describes Dakewe’s mouse lymphocyte separation medium, a density‑gradient solution based on Iodixanol. It is used to isolate lymphocytes from mouse, rat, and rabbit spleens or peripheral blood mononuclear cells with high purity and yield.
The document details the step‑by‑step experimental procedure, including tissue grinding, centrifugation parameters (800 g, 30 min at room temperature), and post‑centrifugation washing. Storage conditions, safety notes, and troubleshooting tips are also provided. This manual is written in Chinese.
Covers: Product Information · Usage Method · Experimental Steps · Precautions · Rat Spleen Cell Separation · Blood Cell Separation
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Dakewe DKW 33-R0100/DKW 33-R0400 Mouse Lymphocyte Separation Medium Manual
User Manual 4 pages · 303 KB · ZHThe Dakewe DKW 33-R0100/DKW 33-R0400 mouse lymphocyte separation medium manual provides preparation, usage, and storage instructions for the…
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in 达科为 manuals
How should the separation medium be stored before and after opening?
Unopened bottles should be kept at 4‑30 °C; after opening, store at 4‑8 °C in sterile conditions and use within two weeks.
From: Dakewe DKW 33-R0100/DKW 33-R0400 Mouse Lymphocyte Separation Medium Manual
What centrifugation settings are recommended for mouse spleen lymphocyte separation?
Centrifuge at 800 g for 30 minutes at room temperature, using a slow acceleration and deceleration (third gear if ten‑step).
From: Dakewe DKW 33-R0100/DKW 33-R0400 Mouse Lymphocyte Separation Medium Manual