The manual details the BIOSS S0202 AgNOR stain kit components, storage conditions, and step‑by‑step staining procedure for paraffin sections.
The BIOSS S0202 AgNOR Stain Kit is designed for research use to detect nucleolar organizer regions (NORs) in tissue sections. The kit includes AgNOR silver solution, AgNOR gel solution, and methyl‑green staining solution, and is supplied as two 50 ml bottles.
The document provides storage guidelines (4 °C, protected from light, 12‑month shelf life), preparation of the working staining solution, a detailed staining protocol for paraffin sections (deparaffinization, staining, optional counter‑stain, dehydration, clearing, and mounting), and safety precautions for laboratory use. This manual is written in Chinese.
| Package size | 2 × 50 ml |
|---|---|
| Shelf life | 12 months |
| AgNOR silver solution volume | 25 ml |
| AgNOR gel solution volume | 25 ml |
| Methyl‑green staining solution volume | 50 ml |
| Recommended section thickness | 3–4 µm |
No, the product is for research use only and not for clinical diagnosis.
Incubate at room temperature for 40–60 minutes.
Sections should be 3–4 µm thick.