Instruction manual for the Bioss C5076 Lowry protein assay kit, describing reagents, procedures, and safety notes.
The Bioss C5076 Lowry Protein Assay Kit provides all reagents needed for protein quantification using the Folin‑phenol (Lowry) method. The kit includes Folin reagent A and B, Folin phenol B reagent, detection buffer, and BSA standard, with storage recommendations for each component.
The manual details step‑by‑step procedures for both microplate (micro‑volume) and spectrophotometric methods, including preparation of standards, sample handling, incubation times, and absorbance measurement at 650 nm. Safety precautions and troubleshooting tips are also provided. This manual is written in Chinese.
| Detection range | 50 µg/ml – 500 µg/ml |
|---|---|
| Folin reagent A storage | 4 °C |
| Folin reagent B storage | 4 °C |
| Folin reagent B stability indicator | Turns deep green when expired |
| Standard protein (BSA) storage | -20 °C |
The kit can accurately measure protein concentrations from 50 µg/ml up to 500 µg/ml.
Folin reagent B should be stored at 4 °C.
Incubate for 30 minutes at 37 °C before measuring absorbance.
Wear a lab coat and disposable gloves during the assay.