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- ZH
- Last added
- 2026-10-03
Featured BIOSS manuals
Bioss C5076 Lowry Protein Assay Kit User Manual
The Bioss C5076 Lowry Protein Assay Kit provides all reagents needed for protein quantification using the Folin‑phenol (Lowry) method. The kit includes Folin reagent A and B, Folin phenol B reagent, detection buffer, and BSA standard, with storage recommendations for each component.
The manual details step‑by‑step procedures for both microplate (micro‑volume) and spectrophotometric methods, including preparation of standards, sample handling, incubation times, and absorbance measurement at 650 nm. Safety precautions and troubleshooting tips are also provided. This manual is written in Chinese.
Covers: Product Information · Product Introduction · Detection Range · Reagents and Storage · Microplate (Micro‑volume) Procedure · Spectrophotometric Procedure
BIOSS S0202 AgNOR Stain Kit User Manual
The BIOSS S0202 AgNOR Stain Kit is designed for research use to detect nucleolar organizer regions (NORs) in tissue sections. The kit includes AgNOR silver solution, AgNOR gel solution, and methyl‑green staining solution, and is supplied as two 50 ml bottles.
The document provides storage guidelines (4 °C, protected from light, 12‑month shelf life), preparation of the working staining solution, a detailed staining protocol for paraffin sections (deparaffinization, staining, optional counter‑stain, dehydration, clearing, and mounting), and safety precautions for laboratory use. This manual is written in Chinese.
Covers: Product Introduction · Product Composition · Storage Conditions · Usage Method · Staining Result · Precautions
BIOSS AK176 Soil Acid Phosphatase Assay Kit User Manual
This kit is designed to detect soil acid phosphatase (S‑ACP) activity using a visible spectrophotometric method. It includes reagents AK176‑A to AK176‑D, a standard solution, and detailed instructions for sample preparation and measurement. The kit is suitable for studying organic phosphorus mineralization and evaluating soil phosphorus bio‑transformation.
The procedure covers enzyme extraction from 0.1 g of air‑dry soil, incubation at 37 °C for 24 h, termination with reagent AK176‑B, centrifugation, and spectrophotometric measurement at 660 nm. Activity is calculated with a provided formula using the standard solution (0.5 µmol mL⁻¹) and the measured absorbances. This manual is written in Chinese.
Covers: Reagent Composition and Storage Conditions · Introduction · Principle · Required Equipment · Crude Enzyme Extraction · Assay Procedure
Latest BIOSS manuals
Bioss C5076 Lowry Protein Assay Kit User Manual
User Manual 2 pages · 267 KB · ZHInstruction manual for the Bioss C5076 Lowry protein assay kit, describing reagents, procedures, and safety notes.
BIOSS S0202 AgNOR Stain Kit User Manual
User Manual 2 pages · 148 KB · ZHThe manual details the BIOSS S0202 AgNOR stain kit components, storage conditions, and step‑by‑step staining procedure for paraffin sections.
BIOSS AK176 Soil Acid Phosphatase Assay Kit User Manual
User Manual 2 pages · 220 KB · ZHThe BIOSS AK176 Soil Acid Phosphatase Assay Kit manual provides reagent composition, storage, assay steps, and calculation method for measuring S‑ACP…
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in BIOSS manuals
What is the detection range of the Bioss Lowry protein assay kit?
The kit can accurately measure protein concentrations from 50 µg/ml up to 500 µg/ml.
At what temperature should Folin reagent B be stored?
Folin reagent B should be stored at 4 °C.
Is the kit intended for clinical diagnosis?
No, the product is for research use only and not for clinical diagnosis.
How long should the tissue be incubated in the AgNOR staining working solution?
Incubate at room temperature for 40–60 minutes.
What equipment is required for the assay?
A visible spectrophotometer, tabletop centrifuge, 37 °C incubator, analytical balance, adjustable pipette, 1 mL glass cuvette, ice, distilled water, ethanol and xylene are needed.
From: BIOSS AK176 Soil Acid Phosphatase Assay Kit User Manual
How is soil acid phosphatase activity calculated?
Activity (nmol d⁻¹ g⁻¹) = [C standard × (A_sample‑A_blank) ÷ (A_standard‑A_blank)] × V_total ÷ W ÷ T, where C = 0.5 µmol mL⁻¹, V_total = 1.45 mL, W is soil mass (g) and T = 24 h.
From: BIOSS AK176 Soil Acid Phosphatase Assay Kit User Manual