The BIOSS AK176 Soil Acid Phosphatase Assay Kit manual provides reagent composition, storage, assay steps, and calculation method for measuring S‑ACP…
This kit is designed to detect soil acid phosphatase (S‑ACP) activity using a visible spectrophotometric method. It includes reagents AK176‑A to AK176‑D, a standard solution, and detailed instructions for sample preparation and measurement. The kit is suitable for studying organic phosphorus mineralization and evaluating soil phosphorus bio‑transformation.
The procedure covers enzyme extraction from 0.1 g of air‑dry soil, incubation at 37 °C for 24 h, termination with reagent AK176‑B, centrifugation, and spectrophotometric measurement at 660 nm. Activity is calculated with a provided formula using the standard solution (0.5 µmol mL⁻¹) and the measured absorbances. This manual is written in Chinese.
| Kit format | 50T/48S |
|---|---|
| Standard solution concentration | 0.5 µmol mL⁻¹ |
| AK176‑A reagent volume | 21 mL per bottle |
A visible spectrophotometer, tabletop centrifuge, 37 °C incubator, analytical balance, adjustable pipette, 1 mL glass cuvette, ice, distilled water, ethanol and xylene are needed.
Activity (nmol d⁻¹ g⁻¹) = [C standard × (A_sample‑A_blank) ÷ (A_standard‑A_blank)] × V_total ÷ W ÷ T, where C = 0.5 µmol mL⁻¹, V_total = 1.45 mL, W is soil mass (g) and T = 24 h.
Store at 4 °C protected from light.