The Life Technologies resDNASEQ® Vero system provides rapid, sensitive qPCR quantitation of Vero host cell DNA with integrated sample preparation.
About this manual
The resDNASEQ® Vero Residual DNA Quantitation System is a qPCR‑based assay for detecting host cell DNA from Vero cells. It combines manual or automated sample preparation with TaqMan® real‑time PCR for reliable, high‑sensitivity quantitation.
Performance data show a limit of quantitation of 1.5 pg/mL, a dynamic range from 3 ng to 30 fg, and assay efficiency of 99.9%. Ordering information and part numbers for the kit and related software are provided in the data sheet.
What's inside
- Overview
- Assay Performance
- Sample Preparation
- Ordering Information
- Specifications
Specifications
| Limit of Quantitation (LOQ) | 1.5 pg/mL |
|---|
| Dynamic Range | 3 ng to 30 fg |
|---|
| PCR Efficiency | 99.9 % |
|---|
| Correlation Coefficient (R²) | 0.999 |
Frequently asked questions
What is the limit of quantitation for the resDNASEQ® Vero system?
The system can reliably quantify DNA down to 1.5 pg per milliliter of sample.
How long does it take to obtain results with the system?
Quantitation of Vero cell DNA is typically completed in less than 4 hours.
Is the resDNASEQ® Vero system intended for clinical diagnostic use?
No, it is for research use only and not intended for animal or human therapeutic or diagnostic applications.
What sample preparation methods are supported?
Both manual and automated sample preparation are available, optimized for quantitative recovery from complex matrices.