User guide for Life Technologies' Universal PCR Master Mix covering RT‑PCR, genotyping, kit contents, instrument compatibility and troubleshooting.
This user guide provides detailed instructions for using the Life Technologies Universal PCR Master Mix in quantitative RT‑PCR and genotyping applications. It includes product description, kit contents, storage conditions, compatible real‑time PCR instruments, and step‑by‑step protocols for assay setup and data analysis.
Additional sections cover troubleshooting, good laboratory practices, chemistry overview, custom assay design, ordering information, and safety considerations, helping users achieve reliable results with the master mix.
| Concentration | 2X |
|---|---|
| Components | AmpliTaq Gold DNA Polymerase, UNG, dNTPs with dUTP, ROX passive reference, optimized buffer |
| Catalog numbers | 4324018, 4364341, 4364343, 4324020, 4326614, 4304437, 4364338, 4364340, 4305719, 4318157, 4326708 |
Each kit includes the 2X Universal PCR Master Mix containing AmpliTaq Gold DNA Polymerase, UNG (unless the no‑UNG version), dNTPs with dUTP, ROX passive reference, and optimized buffer components.
The standard mix includes UNG; the “No AmpErase® UNG” version omits UNG while containing all other ingredients.