Key facts
- Manuals
- 4
- Languages
- EN · ZH
- Last added
- 2026-10-01
Featured Life technologies manuals

Life Technologies Universal PCR Master Mix User Guide
This user guide provides detailed instructions for using the Life Technologies Universal PCR Master Mix in quantitative RT‑PCR and genotyping applications. It includes product description, kit contents, storage conditions, compatible real‑time PCR instruments, and step‑by‑step protocols for assay setup and data analysis.
Additional sections cover troubleshooting, good laboratory practices, chemistry overview, custom assay design, ordering information, and safety considerations, helping users achieve reliable results with the master mix.
Covers: Product Information · Gene expression quantitation · Data Analysis · Genotyping · Troubleshooting · Good Laboratory Practices

Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide
This user guide provides an overview of the Bac-to-Bac® Baculovirus Expression System, a site‑specific transposition method for generating recombinant baculoviruses. It details the required components, storage conditions, and step‑by‑step procedures for cloning, transformation, bacmid isolation, and virus production.
The manual also includes troubleshooting tips, safety information, and appendices with vector maps, ordering details, and support protocols to help researchers efficiently produce high‑level recombinant protein in insect cells.
Covers: Product Information · Description of the system · Experimental outline · Generating the recombinant bacmid · Producing recombinant baculovirus · Expressing recombinant protein

Life Technologies 11802-20 Proteinase K Safety Data Sheet
This Safety Data Sheet (SDS) provides essential safety, handling, and regulatory information for the reagent Proteinase K supplied by Life Technologies under product code 11802‑20. The product is packaged as a bag containing eight 1800 µg portions and is intended for research use only.
The document covers hazard identification, first‑aid measures, fire‑fighting instructions, accidental release procedures, recommended personal protective equipment, storage conditions, exposure limits, physical‑chemical properties, stability, toxicology, ecological impact, disposal, transport, and regulatory compliance. This manual is written in Chinese.
Covers: Identification · Hazard Identification · Composition/Information on Ingredients · First‑Aid Measures · Fire‑Fighting Measures · Accidental Release Measures

Life Technologies resDNASEQ® Vero Residual DNA Quantitation System Datasheet
The resDNASEQ® Vero Residual DNA Quantitation System is a qPCR‑based assay for detecting host cell DNA from Vero cells. It combines manual or automated sample preparation with TaqMan® real‑time PCR for reliable, high‑sensitivity quantitation.
Performance data show a limit of quantitation of 1.5 pg/mL, a dynamic range from 3 ng to 30 fg, and assay efficiency of 99.9%. Ordering information and part numbers for the kit and related software are provided in the data sheet.
Covers: Overview · Assay Performance · Sample Preparation · Ordering Information · Specifications
Latest Life technologies manuals
Life Technologies Universal PCR Master Mix User Guide
User Manual 72 pages · 1.5 MB · ENUser guide for Life Technologies' Universal PCR Master Mix covering RT‑PCR, genotyping, kit contents, instrument compatibility and troubleshooting.
Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide
User Manual 80 pages · 784 KB · ENThe Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide explains how to clone, generate recombinant bacmid, produce baculovirus and…
Life Technologies 11802-20 Proteinase K Safety Data Sheet
Datasheet 7 pages · 160 KB · ZHSafety Data Sheet for Life Technologies 11802-20 Proteinase K (8 × 1800 µg), detailing hazards, handling, storage, and toxicology information.
Life Technologies resDNASEQ® Vero Residual DNA Quantitation System Datasheet
Datasheet 2 pages · 904 KB · ENThe Life Technologies resDNASEQ® Vero system provides rapid, sensitive qPCR quantitation of Vero host cell DNA with integrated sample preparation.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Datasheet
Ratings, dimensions and ordering codes, for selection and design work.
Questions answered in Life technologies manuals
What does the kit contain?
Each kit includes the 2X Universal PCR Master Mix containing AmpliTaq Gold DNA Polymerase, UNG (unless the no‑UNG version), dNTPs with dUTP, ROX passive reference, and optimized buffer components.
Is UNG included in all versions of the mix?
The standard mix includes UNG; the “No AmpErase® UNG” version omits UNG while containing all other ingredients.
What are the catalog numbers for the Bac-to-Bac® system?
The catalog numbers are 10359-016, 10360-014, 10584-027, and 10712-024.
From: Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide
At what temperature should the pFastBac™ vectors be stored?
Store the pFastBac™ vectors at 2°C to 8°C.
From: Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide
What first‑aid measures are recommended for eye contact?
Rinse eyes with plenty of water for several minutes and seek medical advice.
From: Life Technologies 11802-20 Proteinase K Safety Data Sheet
What personal protective equipment should be used when handling Proteinase K?
Wear appropriate gloves, protective clothing, and safety goggles; ensure adequate ventilation.
From: Life Technologies 11802-20 Proteinase K Safety Data Sheet
