The Life Technologies Bac-to-Bac® Baculovirus Expression System User Guide explains how to clone, generate recombinant bacmid, produce baculovirus and…
This user guide provides an overview of the Bac-to-Bac® Baculovirus Expression System, a site‑specific transposition method for generating recombinant baculoviruses. It details the required components, storage conditions, and step‑by‑step procedures for cloning, transformation, bacmid isolation, and virus production.
The manual also includes troubleshooting tips, safety information, and appendices with vector maps, ordering details, and support protocols to help researchers efficiently produce high‑level recombinant protein in insect cells.
| Catalog Numbers | 10359-016, 10360-014, 10584-027, 10712-024 |
|---|---|
| Vector storage temperature | 2°C to 8°C |
| Competent E. coli storage temperature | -85°C to −68°C |
| Transformation efficiency | 1 × 10^8 cfu/μg DNA |
| pFastBac™ vector concentration | 0.5 µg/µL in TE, pH 8.0 |
The catalog numbers are 10359-016, 10360-014, 10584-027, and 10712-024.
Store the pFastBac™ vectors at 2°C to 8°C.
The transformation efficiency is 1 × 10⁸ cfu per microgram of DNA.