This is a laboratory manual that introduces how to use the RayBio® Mouse Netrin-1 ELISA Kit
This manual provides step-by-step instructions for using the RayBio® Mouse Netrin-1 ELISA Kit to quantify mouse Netrin-1 in serum, plasma, and cell culture supernatants. It includes reagent preparation, assay procedure, calculation of results, and troubleshooting guidance. The kit is ISO 13485 certified and designed for research use only.
Storage and handling instructions are provided, including recommended storage conditions for reagents and the importance of avoiding freeze-thaw cycles. The manual also covers standard preparation, dilution protocols, and data analysis using log-log graph paper or software.
| Minimum Detectable Dose | 0.09 ng/ml |
|---|---|
| Intra-Assay CV% | <10% |
The minimum detectable dose is 0.09 ng/ml.
Intra-assay CV% is less than 10%, and inter-assay CV% is less than 12%.
The kit shows no cross-reactivity with Mouse BTLA, BTN1A1, C1ra, Cathepsin L, CD200 R1, CD2F-10, CD38, Cerberus 1, CRISP-4, CXCL17, DCC, DcTRAIL R2, Dkk-2, EG-VEGF, EPCR, EphA3, EphA4, Ephrin-B1, EphrinB2, Frizzled-2, Galectin-4, Galectin-9, GDF-5, GDF-9, GFR alpha-2, GHR, HIN-1, ICAM-5, IGFBP-L1, IL-10 R alpha, IL17 RD, IL-22 R alpha 1, IL-3 R alpha, JAM-B, Latexin, LILRC1, Matrilin-4, Netrin-4, Noggin.