This document is the manual of RayBio® Mouse Osteoactivin IQELISA Kit. It introduces the usage method, storage method, reagent preparation method and other information of the product.
This manual provides step-by-step instructions for using the RayBio® Mouse Osteoactivin IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes reagent preparation, assay procedure, and data calculation methods. The kit is designed for use with real-time PCR instruments and requires specific materials and storage conditions for optimal performance.
The assay involves coating a PCR plate with anti-Mouse Osteoactivin antibody, adding standards and samples, incubating, washing, adding detection reagents, and running qPCR to obtain Ct values for quantification. Results are calculated using standard curves and log-linear regression methods.
| Minimum Quantifiable Dose | 4.9 pg/ml |
|---|---|
| Recovery Rate | 119% |
| Interplate CV | Below 15% |
The minimum quantifiable dose is typically 4.9 pg/ml.
The recovery rate is 119%.
The interplate CV is below 15%.