Agilent VnmrJ 3.1 Quantitative NMR Application Note

About this manual

This document describes a method for performing accurate and precise quantitative Nuclear Magnetic Resonance (NMR) analysis using Agilent Technologies' VnmrJ 3.1 software. It highlights that the integrated intensity of an NMR signal is directly proportional to the number of nuclei, making it a powerful tool without needing specific extinction coefficients.

The note details experimental results obtained on Agilent DirectDrive consoles equipped with OneNMR probes. Using an external standard calibration method (specifically Ibuprofen and Caffeine), the system achieved an accuracy between 96.98% and 102.12% and a precision ranging from 0.59% to 1.07%. The document explains that this approach avoids the contamination issues associated with internal chemical referencing methods. This manual is written in French.

What's inside

• Resume
• Introduction
• Exactitude et la precision de la RMN quantitative
• Donnees experimentales
• Parametres d'acquisition
• Resultats et discussion
• Etalonnage

Specifications

Frequently asked questions

What is the minimum required signal-to-noise ratio for accurate quantification?

A signal-to-noise ratio of at least 150/1 is necessary to reduce integration errors to less than 1%.

What signal-to-noise ratio is required to reduce integration errors to less than 1%?

A signal-to-noise ratio of at least 150/1 is necessary.

What temperature must the sample be maintained at during the quantification experiments?

The sample temperature must be controlled at 25 °C.

How many replicate data sets are needed for each sample in the series?

At least 20 replicates of the data sets must be acquired.