This document describes how to achieve the highest performance of Agilent 1100 Series HPLC system
This document provides a standard check-out procedure to ensure the Agilent 1100 Series HPLC system is free of defects before optimization. It details steps for checking for leaks, contamination, and baseline noise, as well as verifying retention time and area precision using specific parameters and standard samples.
The guide further explains how to optimize performance for specific issues such as low sensitivity, poor precision, low flow rates, and carry-over. It includes recommendations for detector settings, pump configurations, and autosampler adjustments to achieve the best results in normal phase and gradient chromatography.
| Variable Wavelength Detector (VWD) Noise Specification | + 0.75 x 10° AU at 254 nm |
|---|---|
| Diode Array Detector (DAD) Noise Specification | + 1x 10°AU at 254 and 750 nm |
| Retention Time Precision Requirement | < 0.5 % |
| Area Precision Requirement | < 2% |
The recommended flow rate is 1 ml/min.
The optimum reference wavelength is 360 nm with a bandwidth of 100 nm.
A binary pump with a degasser is recommended.
The system should be flushed with water for at least half an hour.