Key facts
- Brand
- Optimizing
- Model
- 1100 Series
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-05
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Agilent 1100 Series HPLC System Optimization Technical Note
This document provides a standard check-out procedure to ensure the Agilent 1100 Series HPLC system is free of defects before optimization. It details steps for checking for leaks, contamination, and baseline noise, as well as verifying retention time and area precision using specific parameters and standard samples.
The guide further explains how to optimize performance for specific issues such as low sensitivity, poor precision, low flow rates, and carry-over. It includes recommendations for detector settings, pump configurations, and autosampler adjustments to achieve the best results in normal phase and gradient chromatography.
Optimizing 1100 Series specifications
| Variable Wavelength Detector (VWD) Noise Specification | + 0.75 x 10° AU at 254 nm |
|---|---|
| Diode Array Detector (DAD) Noise Specification | + 1x 10°AU at 254 and 750 nm |
| Retention Time Precision Requirement | < 0.5 % |
| Area Precision Requirement | < 2% |
Source: Agilent 1100 Series HPLC System Optimization Technical Note
All Optimizing 1100 Series documents by type
Disassembly, diagnostics and part numbers, for repair.
Questions answered in these manuals
What is the recommended flow rate for checking baseline noise?
The recommended flow rate is 1 ml/min.
From: Agilent 1100 Series HPLC System Optimization Technical Note
What is the optimum reference wavelength for a sample with a maximum at 252 nm?
The optimum reference wavelength is 360 nm with a bandwidth of 100 nm.
From: Agilent 1100 Series HPLC System Optimization Technical Note
How can cycle times be significantly increased?
Cycle times must be shortened by using fast gradients with short columns and high flow rates.
From: Optimizing Agilent 1100 Series System for High Sample Throughput Technical Note
What is the effect of switching the autosampler into bypass mode?
Switching the autosampler into bypass mode saves another 300 pl of delay volume, reducing run time.
From: Optimizing Agilent 1100 Series System for High Sample Throughput Technical Note