Agilent Prep-Sil is a preparative-grade liquid chromatography column produced by Agilent. It uses ultra-pure (Type B) silica gel with high specific surface area (400 m2/g) and 100Å pore size in two particle sizes - 5-microns and 10-microns. Agilent Prep-Sil columns have high sample loadability and produce symmetrical peaks even for strongly basic compounds. The columns are stable at pH 0.8-8. Agilent Prep-Sil is especially useful for the preparative purification of acidic, basic, and other highly polar compounds by normal-phase liquid chromatography. Columns are loaded to a stable, uniform bed density using a proprietary, high-pressure slurry-loading technique to give maximum column efficiency and maintain column bed stability.
This document provides a technical overview of Agilent Prep-Sil columns, which are packed with ultra-pure silica suitable for the preparative purification of acidic, basic, and highly polar compounds via normal-phase liquid chromatography. The columns feature high sample loadability and symmetrical peaks, utilizing proprietary slurry-loading techniques to ensure bed stability.
| Particle Size | 5-microns or 10-microns |
|---|---|
| Pore Size | 100A |
| Specific Surface Area | 400 m²/gram |
| pH Stability Range | 0.8-8 |
| Maximum Operating Pressure | 840 bar (5000psi) |
The columns are compatible with water and all common organic solvents.
Avoid use of this column below pH 0.8 or above pH 8.
It is recommended that they be heated in a water bath at 30°C or higher.