Agilent Small RNA kit is a fast and sensitive analysis to resolve small nucleic acids in the size range of 6 to 150 nt, minimal sample consumption
The Agilent Small RNA kit is designed for the Agilent 2100 bioanalyzer to provide fast and sensitive analysis of small nucleic acids ranging from 6 to 150 nt. It allows for the visualization and detection of various RNA species, such as miRNA, siRNA, and t-RNA, down to picogram concentrations within minutes.
Key advantages include high resolution, minimal sample consumption (as little as 50 pg of purified miRNA), and automated analysis completed in about 30 minutes. The kit offers best assay accuracy and precision with pre-packaged reagents and standardized protocols, along with convenient digital data archiving and sharing capabilities.
| Analysis range | 6 - 150 nt |
|---|---|
| Reproducibility of quantitation | 25 % CV (intrachip, for ladder) |
| Quantitative range | 50-2000 pg/μL of purified miRNA in water |
| Sensitivity | 50 pg/μL of 40 nt ladder fragment in water |
| Analysis run time | 30 minutes |
| Number of samples | 11 samples/chip |
| Sample volume | 1 μL |
| Kit stability | 4 months if stored appropriately |
The kit resolves small nucleic acids in the size range of 6 to 150 nt.
As little as 50 pg of purified miRNA is required for analysis in 1 µL volume.
Complete automated analysis of all samples takes about 30 minutes.