This data sheet describes the photometric linearity and range of the Cary 4000, 5000, and 6000i spectrophotometers
This document describes a method to demonstrate the photometric linearity and range of the New Generation Cary 4000/5000/600i spectrophotometers at high absorbance levels. The test utilizes an 'addition of filters' technique, which allows for verification without requiring specific standards.
The document details the materials required, including neutral density screens and blue attenuation filters from part number 9910047700. It provides a step-by-step method involving placing V-holders on cell bases, setting specific instrument parameters (wavelength range 750-450 nm, scan rate 6.0 nm/min), and performing scans with single and combined filters to calculate absorbance error.
Routine linearity tests should be performed using the Linear Neutral Density Kit, part number 9910056100.
Using the standard method parameters, photometric linearity of up to 9 absorbance can be demonstrated.
The standard method parameters will result in a scan time of 45 mins.