Agilent BioHPLC columns can be used for separating and characterizing bio-molecules. It includes four new column families, which can achieve high reproducibility and high resolution analytical separation of antibodies, proteins, peptides and other bio-molecules.
This document introduces Agilent BioHPLC columns, featuring four new column families designed for highly reproducible and high-resolution analytical separations of monoclonal antibodies, proteins, peptides, and other bio-molecules. The portfolio includes Size Exclusion (SEC) and Ion Exchange (IEX) columns tailored for the biopharmaceutical industry.
| Particle size (Bio SEC-3) | 3 um |
|---|---|
| Recommended operating pressure | 135 bar (2,000 psi) |
| Maximum operating pressure | 240 bar (3,500 psi) |
| Recommended temperature range | 10 to 30°C |
| Maximum temperature | 80°C |
| Recommended buffer | 150 mM phosphate buffer, pH 7.0 |
The recommended flow rate is 0.1 to 1.25 mL/min.
The maximum operating temperature is 80°C for both column types.
The recommended operating pressure is 135 bar (2,000 psi).
The maximum pressure limit is 240 bar (3,500 psi).