This document introduces the method of using the 2100 bioanalyzer and Protein 200 Plus Assay to control the quality of antibodies.
This document describes a study evaluating the suitability of the Agilent 2100 bioanalyzer for quality control of antibodies. The system was used to analyze two different antibody samples under reducing conditions using the Protein 200 Plus LabChip kit and software assay. The study aimed to verify the technology's ability to monitor protein identification, quantitation, purity, and stability.
The system allows sizing and analysis of proteins ranging from 5 to 200 kDa, depending on the application.
The Protein 200 Plus assay uses a minimum peak height of 0.1 and a minimum peak width of 0.1.
The kit includes a total of 25 chips along with spin filters and reagents.