This document is the operating manual for the Olympus BX51 microscope, which describes the usage of different modes of the microscope, including brightfield, fluorescence, phase contrast, Nomarski differential interference contrast (DIC), simultaneous fluorescence and phase contrast, simultaneous fluorescence and DIC, and polarized light. The manual provides detailed instructions on the steps to operate the microscope, such as turning on the light, adjusting brightness, adjusting interpupillar distance, placing slides, finding and focusing on specimens.
This manual covers the setup and operation of the Olympus BX51 microscope for a variety of imaging techniques. It includes detailed procedures for brightfield, fluorescence, phase‑contrast, Nomarski differential interference contrast (DIC), simultaneous fluorescence‑phase contrast, simultaneous fluorescence‑DIC, and polarized light imaging, with instructions on adjusting light intensity, condenser settings, and objective selection.
Safety and maintenance guidelines are also provided, describing how to turn off the halogen and mercury light sources, clean the 100× objective with lens paper only, and keep the work area clean and tidy after each session.
| Fluorescence light source | Mercury bulb |
|---|---|
| Transmitted light source | Halogen lamp |
Clean the 100× objective carefully with lens paper only; do not use Kimwipes.