The document introduces a product with specific features and characteristics. The product has high performance, strong reliability, and ease of use.
Super Taq DNA Polymerase is a high‑fidelity enzyme that combines thermostable Taq polymerase with 3'‑5' exonuclease activity, delivering high amplification efficiency and low mismatch rates. The product is supplied as a 5 U/µl solution with a purity greater than 99 % and is suitable for PCR and TA cloning.
The manual describes the enzyme’s composition, storage buffer, and recommended reaction setup, including buffer components, dNTP concentration, primer amounts, and cycling parameters. It also outlines usage precautions such as the need to add the enzyme to primers immediately because of its exonuclease activity, and provides tips for amplifying high‑GC or difficult templates. This manual is written in Chinese.
| Enzyme concentration | 5 U/µl |
|---|---|
| Purity | >99 % |
| Storage temperature | -20 °C |
One activity unit (U) is the amount of enzyme that incorporates 10 nmol dNTP in 30 minutes at 74 °C.
The enzyme should be stored at –20 °C.
Yes, it has 3'‑5' exonuclease activity that can degrade primers, so the enzyme should be mixed with primers immediately before the reaction.