GENMED Recombinant Adenovirus Transfection Reagent is a purposed product to help adenoviral genome plasmid or adenoviral vector shuttle plasmid DNA easily pass the viral packaging cells through strong cationic liposomal delivery vector. It is suitable for various virus plasmid DNA (e.g. pJM17 or pBHG10) transfer to various types of adherent viral packaging cell lines (e.g. human embryonic retina 911 cells or human embryonic kidney 293 cells). It can be used for stable transfection or transient transfection.
This manual describes the purpose and technical background of the GENMED GMS60036 recombinant adenovirus transfection kit, which uses a cationic lipid carrier to deliver adenoviral genome plasmids into packaging cells such as 911 or 293. It lists the kit components (Reagent A–D) and required user‑supplied items like plasmid DNA and cell culture vessels.
Detailed experimental steps are provided, covering preparation of the transfection mix, cell handling, incubation conditions, and post‑transfection processing. The document also includes storage instructions, quality standards, ordering information, and contact details for technical support. This manual is written in Chinese.
| Reagent A (Cleaning Solution) volume per kit | 5 mL |
|---|---|
| Reagent B (Transfection Solution) volume per kit | 200 µL |
| Reagent C (Enhancement Solution) volume per kit | 1 mL |
| Reagent D (Storage Solution) volume per kit | 100 mL |
| Number of transfections per kit | 10 |
| Recommended storage temperature | 4 °C |
Store the kit at 4 °C in a refrigerator, where it remains effective for six months.
The kit is compatible with adherent virus‑packaging cell lines such as 911 cells and 293 cells.
One kit provides reagents for up to ten transfection experiments.