Key facts
- Manuals
- 12
- Categories
- 1
- Languages
- ZH
- Last added
- 2012-03-13
Featured Shanghai GENMED manuals
GENMED QuikFusin Cell Transfection Kit User Manual
The QuikFusin kit is a non‑liposomal transfection reagent designed for high‑efficiency, low‑toxicity delivery of DNA fragments (including >12 kb) and oligonucleotides into over 700 adherent and suspension cell lines. The kit includes Reagent A (transfection solution) and Reagent B (mediating solution) that are mixed immediately before use.
The manual outlines the preparation of the transfection mixture, the recommended cell density (≈1 × 10⁶ cells per 25 cm² flask), DNA amount (7–9 µg), incubation times (15 min at room temperature, then 3 h at 37 °C), and post‑transfection handling, including optional selection steps for stable expression. Storage, quality standards, and ordering information are also provided. This manual is written in Chinese.
Covers: Purpose · Technical Background · Product Contents · Storage · Experimental Procedure · Precautions
GENMED GMS60036 Recombinant Adenovirus Transfection Kit User Manual
This manual describes the purpose and technical background of the GENMED GMS60036 recombinant adenovirus transfection kit, which uses a cationic lipid carrier to deliver adenoviral genome plasmids into packaging cells such as 911 or 293. It lists the kit components (Reagent A–D) and required user‑supplied items like plasmid DNA and cell culture vessels.
Detailed experimental steps are provided, covering preparation of the transfection mix, cell handling, incubation conditions, and post‑transfection processing. The document also includes storage instructions, quality standards, ordering information, and contact details for technical support. This manual is written in Chinese.
Covers: Main Use · Technical Background · Product Contents · Storage · User‑Supplied Materials · Experimental Procedure
GENMED Large Amount Lipofusin Kit User Manual
This manual describes the purpose and technical principle of the GENMED Large Amount Lipofusin transfection kit, which includes Reagent A (cleaning solution), Reagent B (transfection solution) and Reagent C (carrier solution). The kit is designed for high‑efficiency delivery of DNA, RNA or other nucleic acids into a wide range of cell types.
The document outlines storage conditions, detailed procedural steps, and important precautions such as using filtered tips and avoiding cell loss during liquid changes. Contact information for technical support and warranty terms are also provided. This manual is written in Chinese.
Covers: Main Use · Technical Principle · Kit Contents · Storage Conditions · Procedure · Precautions
GENMED GM-30010v.A Gel Electrophoresis Kit Product Manual
The manual describes the GENMED Gel Electrophoresis Kit, which uses tricine (麦黄酮) to replace glycine for improved separation of small‑molecule proteins and peptides ranging from 4 kDa to 30 kDa. It lists all reagents (Reagents A‑I) and required consumables, and gives storage recommendations for each component.
Detailed experimental steps cover gel preparation, sample handling, electrophoresis running conditions, and post‑run staining. Precautions, quality standards, and ordering information are also provided, along with contact details for technical support. This manual is written in Chinese.
Covers: Purpose · Technical Background · Product Contents · Storage · Experimental Procedure · Sample Preparation
Latest Shanghai GENMED manuals
GENMED QuikFusin Cell Transfection Kit User Manual
User Manual 3 pages · 127 KB · ZHThe GENMED QuikFusin Cell Transfection Kit User Manual provides step‑by‑step instructions, reagent details, and safety notes for transfecting various cell…
GENMED GMS60036 Recombinant Adenovirus Transfection Kit User Manual
User Manual 4 pages · 117 KB · ZHThe GENMED GMS60036 manual provides usage instructions, component list, storage guidelines, and safety notes for the recombinant adenovirus transfection kit.
GENMED Large Amount Lipofusin Kit User Manual
User Manual 3 pages · 121 KB · ZHThe GENMED Large Amount Lipofusin Kit User Manual provides step‑by‑step instructions, reagent details, and safety information for transfecting cells with…
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GENMED GM-30010v.A Gel Electrophoresis Kit Product Manual
User Manual 5 pages · 139 KB · ZHThe GENMED GM-30010v.A Gel Electrophoresis Kit manual provides usage instructions, reagent list, and experimental procedures for separating 4‑30 kDa proteins.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Shanghai GENMED manuals
How many transfections can be performed with one QuikFusin kit?
One kit provides enough reagents for 10 transfections.
What DNA amount should be used per transfection?
Use 7 to 9 µg of DNA for each transfection.
How should the kit be stored?
Store the kit at 4 °C in a refrigerator, where it remains effective for six months.
From: GENMED GMS60036 Recombinant Adenovirus Transfection Kit User Manual
Which cell lines can be used with this kit?
The kit is compatible with adherent virus‑packaging cell lines such as 911 cells and 293 cells.
From: GENMED GMS60036 Recombinant Adenovirus Transfection Kit User Manual
How many runs can the kit perform?
The kit supports either 10 runs with 30 mL gel volume or 60 runs with 5 mL gel volume.
From: GENMED GM-30010v.A Gel Electrophoresis Kit Product Manual
What voltage and time are recommended for electrophoresis?
Run at 100 V for about 15 hours (15 cm gel length) or at 200 V for roughly 5 hours.
From: GENMED GM-30010v.A Gel Electrophoresis Kit Product Manual