This document describes the features and characteristics of HD Biosciences Co., Ltd's Human Histamine H3 Receptor Cell Line product. The cell line expresses the H3 receptor and couples to G protein, mediating intracellular calcium ion mobilization. This product is suitable for studying the effects of agonists and antagonists of the histamine H3 receptor.
This manual describes the HD Biosciences CH2098-3 human histamine H3 receptor cell line, including its generation, genetic construct, and intended use for calcium‑based functional assays. It outlines the cell line’s characteristics, such as host cell (HEK293), passage number shipped, and antibiotic selection.
Key sections cover cell line information, recommended culture media and supplements, detailed assay protocol for calcium signaling, results interpretation, and lists of reagents, media formulations, and preparation instructions for trypsin‑EDTA and culture medium.