This manual provides installation, usage, and maintenance instructions for the Waters Gen-Pak FAX anion-exchange HPLC column used for nucleic acid analysis.
The Waters Gen-Pak FAX column is a 4.6 x 100 mm steel column containing a polymer-based high-performance anion-exchange packing with 2.5 µm particles. It is designed for high-resolution analysis and purification of various nucleic acid species, including DNA restriction fragments, PCR products, plasmids, and synthetic oligonucleotides, up to at least 5,000 base pairs.
| Particle Size | 2.5 µm |
|---|---|
| Column Dimensions | 4.6 x 100 mm |
| Maximum Operating Pressure | 4,000 psi |
| pH Range | 1.5 to 12 |
| Recommended Flow Rate | 0.75 mL/min |
| Recommended Temperature | 30°C |
| Recommended Buffer A | 25 mM Tris/Cl, 1 mM EDTA, pH 8.0 |
| Recommended Buffer B | 25 mM Tris/Cl, 1 mM EDTA, 1.0 M NaCl, pH 8.0 |
Do not exceed 4,000 psi operating pressure or about 1 mL/min at 25 °C.
Stay within a pH range of about 1.5 to 12, avoiding concentrated acids or bases.
Clean the column with 3 to 5 mL of 20 - 40% acetic acid.
Flush with water and methanol/water mixture, disconnect, plug ends, and store at 4 °C.