This manual provides instructions for the preparation, use, and maintenance of Sunresin SeplifeG100M dextran gel filtration medium for protein purification.
The document describes the SeplifeG100M product as a high-molecular compound with a three-dimensional network structure used for protein separation, molecular weight determination, equilibrium constant measurement, and cell separation. It explains that larger molecules are eluted first while smaller molecules are retained longer due to pore size exclusion. This manual is written in Chinese.
| Particle Size (Dry) | 40～120 μm |
|---|---|
| Swelling Degree | 15~20 |
| Separation Range | 4000~10×104 |
| pH Stability | 2~10 |
The general recommendation is 5% of the bed volume, though we suggest controlling initial loading to 1-2%.
The gel should undergo one CIP after every 10 uses to remove precipitated and stubborn residual proteins.