This manual provides instructions for the preparation, operation, and maintenance of the Sunresin Seplife G50 M chromatography medium for desalting and…
The Sunresin Seplife G50 M is a chromatography medium with a three-dimensional network structure made from dextran cross-linked with epichlorohydrin. It is designed for applications such as desalting, peptide separation, cleaning biological extracts, and molecular weight determination. The medium allows for the separation of molecules based on size, where larger molecules elute first and smaller molecules elute last. This manual is written in Chinese.
| Appearance | White spherical, odorless and tasteless |
|---|---|
| Particle Size | 50~150 μm (dry) |
| Swelling Degree | 9~11 |
| Separation Range | 1500~3×10^4 |
| pH Stability (Long term) | 2~10 |
| pH Stability (Short term/CIP) | 2~13 |
In distilled water at room temperature, it generally requires 1-2 days to swell fully.
The medium should undergo CIP after being used 10 times to remove precipitated proteins.
The loading volume is generally 5% of the column bed volume, though we suggest starting with 1-2%.
Dry powder should be stored at 4~30°C in a dry, ventilated, and clean place.