This manual provides instructions for the ShineGene ShinoRec one-step directional cloning kit, detailing product composition, primer design, recombination…
The ShineGene ShinoRec® One-Step Directional Cloning Kit (Seamless Cloning Kit) is a gene cloning technology that does not rely on ligase. It uses homologous recombination to seamlessly link target fragments with vectors without being limited by restriction enzyme sites. The kit features simple operation and a reaction time as short as 20 minutes, achieving a positive rate of over 95%. This manual is written in Chinese.
| Reaction Time | 20 minutes |
|---|---|
| Homologous Sequence Length | 15-20 bp |
| Minimum Vector Concentration | >15 ng/μl |
| Competent Cell Efficiency | >5×10^6 cfu/μg |
No, it is a technology that does not rely on ligase.
Yes, both single and double digestions are applicable to the kit.
Increase homologous sequence length, increase molar ratio, extend reaction time, or save remaining liquid for subsequent transformation.