This guide provides instructions for preparing up to 384 dual-indexed paired-end libraries from DNA using the Illumina DNAPrep workflow.
The Illumina DNAPrep Reference Guide details a protocol for preparing up to 384 dual-indexed paired-end libraries from DNA using the Illumina DNAPrep workflow. The protocol utilizes tagmentation and enzymatic reactions to fragment DNA and add adapter sequences in approximately 15 minutes. It is compatible with DNA inputs ranging from 1 to 500 ng or higher, including whole blood and saliva samples when using specific lysis protocols.
| Maximum library yield | 384 dual-indexed paired-end libraries |
|---|---|
| Minimum DNA input | 1 ng |
| Tagmentation time | 15 minutes |
| Compatible DNA input range | 1–500 ng or higher |
| Recommended DNA purity ratio (260/280) | 1.8–2.0 |
| Recommended DNA purity ratio (260/230) | 2.0–2.2 |
| PCR amplicon minimum size | >150 bp |
The protocol requires as little as 1 ng of DNA input.
Yes, it can prepare libraries directly from whole blood or saliva samples when using an extraction protocol.
The protocol is optimized for DNA with a 260/280 absorbance ratio of 1.8–2.0.
The tagmentation and enzymatic reaction to fragment DNA and add adapter sequences takes only 15 minutes.