The IDEXX Enterolert test kit manual provides step‑by‑step instructions for detecting Enterococcus in water samples.
Enterolert is a reagent kit for detecting Enterococcus (including E. faecalis and E. faecium) in water. It uses IDEXX’s patented fixed‑enzyme substrate technology (DST) that produces fluorescence when β‑glucosidase metabolizes the nutrient substrate.
The manual describes both qualitative (presence/absence) and quantitative (MPN) procedures, result interpretation, storage conditions, safety precautions, and quality‑control steps. It also lists the required incubation temperature, detection time, and UV observation guidelines. This manual is written in Chinese.
| Detection limit | 1 cfu/100 ml |
|---|---|
| Incubation temperature | 41 °C ±0.5 °C |
| Incubation time | 24 hours (results readable 24‑28 h) |
| Storage temperature | 2‑30 °C, protected from light |
| UV observation wavelength | 365 nm (6‑watt) |
Results are readable within 24‑28 hours after incubation.
The kit can detect as few as 1 colony‑forming unit per 100 ml of sample.
Incubate the samples at 41 °C ±0.5 °C for 24 hours.
Use a 6‑watt, 365 nm UV lamp and observe the sample in a dark environment.