The GenXion JXF60162 TUNEL Apoptosis Detection Kit manual provides step‑by‑step instructions, reagent list, and safety notes for detecting apoptosis in…
The kit enables high‑sensitivity, rapid in‑situ detection of apoptosis in both tissue sections (paraffin‑embedded or frozen) and cell preparations. Detection relies on TdT‑mediated incorporation of a fluorescent dUTP into DNA breaks, which can be visualized by fluorescence microscopy.
The manual details sample fixation, permeabilization, enzyme reactions, and labeling steps, including preparation of positive and negative controls, storage conditions (‑20 °C for up to one year), and safety precautions such as wearing a lab coat and disposable gloves. This manual is written in Chinese.
| Test quantity | 50 |
|---|---|
| Storage temperature | -20 °C |
| Fluorescence excitation wavelength | 495 nm |
| Fluorescence emission wavelength | 519 nm |
Mix 35 µL balance solution (1×), 10 µL fluorescence labeling solution, and 5 µL TdT enzyme, vortex, and use immediately; incubate at 37 °C in the dark for 60 min.
Store the kit at –20 °C for up to one year; keep the fluorescence labeling solution protected from light.