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GenXion JXF60162 TUNEL Apoptosis Detection Kit User Manual
The kit enables high‑sensitivity, rapid in‑situ detection of apoptosis in both tissue sections (paraffin‑embedded or frozen) and cell preparations. Detection relies on TdT‑mediated incorporation of a fluorescent dUTP into DNA breaks, which can be visualized by fluorescence microscopy.
The manual details sample fixation, permeabilization, enzyme reactions, and labeling steps, including preparation of positive and negative controls, storage conditions (‑20 °C for up to one year), and safety precautions such as wearing a lab coat and disposable gloves. This manual is written in Chinese.
Covers: Product Overview · Detection Principle · Reagents List · Procedure · Positive and Negative Controls · Storage Conditions
Latest GenXion manuals
GenXion JXF60162 TUNEL Apoptosis Detection Kit User Manual
User Manual 3 pages · 278 KB · ZHThe GenXion JXF60162 TUNEL Apoptosis Detection Kit manual provides step‑by‑step instructions, reagent list, and safety notes for detecting apoptosis in…
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in GenXion manuals
How is the TdT enzyme reaction mixture prepared?
Mix 35 µL balance solution (1×), 10 µL fluorescence labeling solution, and 5 µL TdT enzyme, vortex, and use immediately; incubate at 37 °C in the dark for 60 min.
From: GenXion JXF60162 TUNEL Apoptosis Detection Kit User Manual
What are the storage conditions for the kit?
Store the kit at –20 °C for up to one year; keep the fluorescence labeling solution protected from light.
From: GenXion JXF60162 TUNEL Apoptosis Detection Kit User Manual