GE Healthcare Sephacryl S-100 to S-500 High Resolution Instructions

About this manual

This document provides detailed instructions for the preparation of Sephacryl™ HR gel filtration media suspensions and the subsequent packing of chromatography columns. It outlines a specific two-step packing method designed to ensure the medium is most tightly packed at the top of the column, thereby improving resolution compared to traditional methods.

What's inside

• Introduction
• To prepare the medium suspension
• To pack the column
• Recommended packing flow rates
• Efficiency test (plate number)
• Medium characteristics

Frequently asked questions

What is the recommended procedure to improve resolution when packing the column?

You should pack the column in two steps, then insert a second adaptor at the top, and finally turn the column upside-down so the sample is applied in the most tightly packed zone.

How much medium slurry should be measured out before diluting it with eluent buffer?

You must measure out a volume of medium slurry that is 1.5 times the desired packed medium volume.

What are the recommended packing flow rates for an XK 26/70 column using aqueous buffers at room temperature?

The recommended flow rate is 150 ml/h for the first step and 300 ml/h for the second step.