A technical publication describing protein purification with the ÄKTAxpress system and high‑content cell analysis using the IN Cell Analyzer 2000.
The document presents methods for automated multi‑step purification of recombinant tagged proteins using the ÄKTAxpress chromatography system. It details three‑step and four‑step protocols that combine affinity, desalting, ion‑exchange, and gel‑filtration chromatography without operator intervention, and reports yields and purity for proteins such as DNaseE7/Im7 and hWO‑2 Fab.
It also introduces GE Healthcare’s high‑content analysis platform, focusing on the IN Cell Analyzer 2000. The instrument provides rapid whole‑plate imaging (≈200 images per 96‑well plate in ~3 min) and advanced deconvolution for improved contrast, supporting applications such as stem‑cell clone analysis, tumor‑cell assays, and zebrafish embryo studies. Software tools for data analysis and management are described as well.
| ÄKTAxpress flow rate (IMAC column) | 1 ml/min |
|---|---|
| IN Cell Analyzer 2000 imaging speed | ≈200 images per 96‑well plate in ~3 min |
Approximately 200 images can be captured in about three minutes.