Protocol for using Clontech TALONspin™ Columns to purify his‑tagged proteins under native or denaturing conditions.
The TALONspin™ Columns protocol describes how to purify small amounts of his‑tagged proteins quickly using spin‑column technology. Each column contains 0.5 ml of TALON‑NX resin and is intended for single‑use applications, yielding 2–4 mg of purified protein.
The document covers buffer preparation, native and denaturing protein extraction, step‑by‑step purification procedures, and a troubleshooting guide. Detailed centrifugation speeds, buffer compositions, and optional reagents such as ProteoGuard™ protease inhibitor are provided to ensure optimal performance.
| Resin volume per column | 0.5 ml |
|---|---|
| Typical protein yield per column | 2–4 mg his‑tagged protein |
| Catalog numbers | 635601, 635602, 635603 |
Approximately 2–4 mg of his‑tagged protein can be recovered per column, depending on expression level and lysate concentration.
The protocol recommends centrifuging the column at 700 x g for 2 minutes during washing and elution steps.