The manual details the protocol for constructing acceptor vectors with the CreatorTM kit, including components, primer design, PCR steps, and troubleshooting.
The CreatorTM Acceptor Vector Construction Kit provides all reagents needed to convert conventional expression vectors into Creator‑compatible acceptor vectors. Using Cre‑loxP recombination, the kit enables rapid construction of expression vectors for a variety of research applications.
The document walks through the list of components, required additional materials, general laboratory considerations, primer design guidelines, step‑by‑step construction procedures, and a troubleshooting guide. It also includes references and a list of related Clontech products.
| AdvanTaq DNA Polymerase concentration | 50X |
|---|---|
| Expected restriction fragment size | 161 bp |
| Glycerol content in reaction mix | 50 % |
A fragment of 161 bp should be released when analyzed on a 2 % agarose gel.
Increase the number of PCR cycles by 3–5 and verify that all components are functional using a positive control.