Key facts
- Manuals
- 2
- Languages
- EN
- Last added
- 2026-10-03
Featured Clontech manuals
Clontech CreatorTM Acceptor Vector Construction Kit User Manual
The CreatorTM Acceptor Vector Construction Kit provides all reagents needed to convert conventional expression vectors into Creator‑compatible acceptor vectors. Using Cre‑loxP recombination, the kit enables rapid construction of expression vectors for a variety of research applications.
The document walks through the list of components, required additional materials, general laboratory considerations, primer design guidelines, step‑by‑step construction procedures, and a troubleshooting guide. It also includes references and a list of related Clontech products.
Covers: Introduction & Protocol Overview · List of Components · Additional Materials Required · General Considerations · Primer Design · Acceptor Vector Construction Procedure
Clontech TALONspin™ Columns Protocol
The TALONspin™ Columns protocol describes how to purify small amounts of his‑tagged proteins quickly using spin‑column technology. Each column contains 0.5 ml of TALON‑NX resin and is intended for single‑use applications, yielding 2–4 mg of purified protein.
The document covers buffer preparation, native and denaturing protein extraction, step‑by‑step purification procedures, and a troubleshooting guide. Detailed centrifugation speeds, buffer compositions, and optional reagents such as ProteoGuard™ protease inhibitor are provided to ensure optimal performance.
Covers: Introduction · Buffer Preparation · Protein Extraction Protocols · Protein Purification Protocol · Troubleshooting Guide
Latest Clontech manuals
Clontech CreatorTM Acceptor Vector Construction Kit User Manual
User Manual 19 pages · 336 KB · ENThe manual details the protocol for constructing acceptor vectors with the CreatorTM kit, including components, primer design, PCR steps, and troubleshooting.
Clontech TALONspin™ Columns Protocol
User Manual 5 pages · 401 KB · ENProtocol for using Clontech TALONspin™ Columns to purify his‑tagged proteins under native or denaturing conditions.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Clontech manuals
What fragment size should appear after digestion of the construct?
A fragment of 161 bp should be released when analyzed on a 2 % agarose gel.
From: Clontech CreatorTM Acceptor Vector Construction Kit User Manual
What should I do if no PCR product is observed?
Increase the number of PCR cycles by 3–5 and verify that all components are functional using a positive control.
From: Clontech CreatorTM Acceptor Vector Construction Kit User Manual
How much his‑tagged protein can be obtained from one TALONspin column?
Approximately 2–4 mg of his‑tagged protein can be recovered per column, depending on expression level and lysate concentration.
What centrifugation speed is recommended for TALONspin column steps?
The protocol recommends centrifuging the column at 700 x g for 2 minutes during washing and elution steps.