Beyotime C1995S Cell Plasma Membrane Staining Kit with DiD User Manual

This manual provides instructions for using the Beyotime C1995S kit to stain cell plasma membranes with far-red fluorescence using the DiD probe.


Beyotime C1995S Cell Plasma Membrane Staining Kit with DiD User Manual - cover page
Brand
Beyotime Biotechnology
Document type
User Manual
Language
Chinese
Pages
3
File format
PDF
File size
525 KB
Published
21 October, 2026
Updated
21 October, 2026
MD5 checksum
DDF9DB9FBAF12B07CEA7B4740AC685FE

About this manual

The Beyotime Biotechnology Cell Plasma Membrane Staining Kit (DiD), product number C1995S, is designed for rapid and sensitive staining of cell plasma membranes. It utilizes DiIC 18(5) as a lipid-soluble carbocyanine dye that diffuses laterally within the membrane to provide stable far-red fluorescence labeling suitable for live or fixed cells. This manual is written in Chinese.

What's inside

  • Product Introduction
  • Storage Conditions
  • Solution Preparation
  • Suspension Cell Staining
  • Adherent Cell Staining
  • Fixation and Permeabilization
  • Staining of Fixed Cells or Sections

Specifications

Excitation Wavelength (Max)644nm
Emission Wavelength (Max)665nm
Storage Temperature-20ºC

Frequently asked questions

What is the recommended staining time for live cells?

The staining time typically ranges from 5 to 20 minutes.

What is the maximum number of times the C1995S kit can be used for flow cytometry?

When the detection system volume is 0.5ml per sample, the kit can perform 200 detections.

What is the recommended incubation time for staining live cells with this kit?

The staining time is typically 5 to 20 minutes at 37ºC in the dark.

What are the maximum excitation and emission wavelengths for DiD?

The maximum excitation wavelength is 644nm and the maximum emission wavelength is 665nm.

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