This manual describes the Beyotime D0508 kit for detecting mutations in genome-edited DNA using T7 Endonuclease I, including extraction, PCR, and analysis…
The Beyotime D0508 Genome-Editing Mutation Detection Kit is a simple, reliable, and rapid reagent system based on T7 Endonuclease I (T7EI) for detecting mutations in genomic DNA after gene editing. It provides a complete set of reagents for one-step genomic DNA extraction, high-fidelity PCR amplification, denaturation/annealing, and T7EI digestion, along with positive controls. The kit is designed to detect insertions or deletions (indels) caused by nucleases such as CRISPR/Cas9, TALEN, or ZFN. This manual is written in Chinese.
| Detection Principle | Based on T7 Endonuclease I recognition of heteroduplex DNA |
|---|---|
| Storage Temperature | -20 ºC |
| Shelf Life | At least one year |
The kit should be stored at -20 ºC and has a validity of at least one year.
No, the PCR amplification products can be directly used for denaturation/annealing and T7EI digestion without additional purification.
The digestion is typically sufficient after 15 minutes at 37 ºC, though it can be extended up to 60 minutes.