Technical data sheet for Akoya Biosciences' MOTiF PD-1/PD-L1 Panel: Auto Melanoma Kit for detecting six protein biomarkers on melanoma FFPE sections using…
This document provides technical information for the MOTiF PD-1/PD-L1 Panel: Auto Melanoma Kit, designed for detecting six protein biomarkers on melanoma FFPE sections using the Opal™ method. It is optimized for use with the Leica BOND™ RX and compatible with PhenoImager HT and PhenoImager Fusion imaging systems. The kit includes ready-to-use antibodies for FoxP3, PD-L1, Sox10/S100, PD-1, CD8, and CD68, with specific fluorophore targets and storage instructions. Safety and quality control information are also provided.
| Target Color | FoxP3 Opal 570 in Yellow |
|---|---|
| Nucleus Counterstain | DAPI in Blue |
The Opal workflow allows for simultaneous detection of multiple biomarkers in tissue. This Opal protocol was written specifically for the MOTiF PD-1/PD-L1 Panel: Auto Melanoma Kit on formalin fixed paraffin embedded (FFPE) tissue using the Leica BOND™ RX to stain and detect FoxP3, PD-L1, Sox10/S100, PD-1, CD8, and CD68 on melanoma FFPE sections.
Store dry Opal reagents at -20°C. Upon reconstitution in DMSO, store at 2–8°C. Store remaining kit components at 2–8°C.