GV3101 chemically competent Agrobacterium cells contain the pCAMBIA2301 plasmid and include a detailed transformation protocol.
GV3101 is a C58‑type Agrobacterium strain engineered with rifampicin resistance (rif) and gentamicin resistance (gent). The cells are supplied as 100 µl aliquots at –80 °C and include the control vector pCAMBIA2301 (10 ng/µl). The product achieves a transformation efficiency greater than 10⁴ cfu/µg DNA.
The document provides a step‑by‑step transformation procedure, antibiotic formulations, and media recipes (LB and YEB). It also lists common antibiotic resistance profiles for Agrobacterium strains and important handling precautions such as limiting plasmid volume to 10 % of the competent cell volume and avoiding excessive rifampicin concentrations. This manual is written in Chinese.
| Aliquot volume | 100 µl per vial |
|---|---|
| Control vector | pCAMBIA2301 (10 ng/µl) |
| Storage condition | -80 °C for 12 months |
| Transformation efficiency | >10⁴ cfu/µg DNA |
Store the cells at –80 °C, where they remain viable for up to 12 months.