This manual introduces the use of GMO Hunter SS kit produced by HaiKang Life Sciences Co., Ltd. This kit is used to detect Soy Specific gene sequence in soybeans.
The GMO Hunter SS operating manual describes the composition of the soy‑specific real‑time PCR reagent kit and the proper storage conditions. It details the preparation of reaction mixes, recommended volumes, and the need for positive and negative controls.
The document outlines the PCR cycling program, data collection settings, and guidelines for interpreting results. Technical support contact information and warranty terms are also provided for users who need assistance. This manual is written in Chinese.
| DNA template amount per reaction | 100‑200 ng |
|---|---|
| PCR cycling program | 50°C 2 min, 95°C 10 min, then 40 cycles of 95°C 15 s and 60°C 1 min |
Run an initial step of 50 °C for 2 min, then 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 60 °C for 1 min, collecting data at the FAM channel.