This manual describes the method of using the Hunter 18S fluorescence real-time PCR kit produced by Haikang Life Technology Co., Ltd. to detect 18S gene sequence.
The GMO Hunter 18S manual provides step‑by‑step instructions for preparing and running the 18S real‑time PCR assay. It details reagent composition, storage requirements, and the PCR cycling program required for accurate detection.
Additional sections cover control setup, data analysis guidance, warranty information, and contact details for technical support from Haikang Life Technology. This manual is written in Chinese.
| Reagent storage temperature | -20 °C |
|---|---|
| Mastermix volume per reaction | 11.2 µL |
| Water volume per reaction | 8.8 µL (adjusted for template) |
| Fluorescence detection wavelength | 530 nm (FAM) |
Reagents and positive controls must be stored at –20 °C and kept protected from light, for example by wrapping tubes in aluminum foil.
Each sample should be run in two PCR replicates, with a positive control (2 µL) and a negative control (2 µL water) included in every run.