This document is a revised version of the operating manual for GMO Hunter NK603 gene sequence qualitative fluorescence real-time PCR. It describes the composition of the product's reagent kit, storage conditions, and operating procedures.
This manual describes the composition of the NK603 real‑time PCR reagent kit and the complete operating procedure for detecting the NK603 GMO sequence. It includes reagent volumes, storage requirements, PCR cycling parameters, and guidelines for setting positive and negative controls.
The document also covers storage conditions, warranty terms, and technical support contacts for Haikang Life Technology. Users are instructed to store reagents at -20 °C, protect them from light, and follow the recommended PCR program to obtain reliable results. This manual is written in Chinese.
| Mastermix storage temperature | -20 °C |
|---|---|
| Positive control volume | 2 µL |
| Negative control volume | 2 µL water |
| DNA template per reaction | 100‑200 ng |
| PCR cycling program | 95 °C 2 min, 95 °C 10 min, 40 cycles of 95 °C 15 s / 60 °C 60 s |
Reagents must be kept at –20 °C and protected from light, for example by wrapping the tubes in aluminum foil.
Each reaction should include a positive control (2 µL) and a negative control (2 µL water).