Modern Molecular Biology Experimental Principle and Technology is a book published by Science Press, co-edited by Chen Defu and Chen Xiwen. This book is illustrated, innovative and easy to understand, which is a modern molecular biology experimental technology textbook and reference book suitable for the development of 21st century life science. The whole book is divided into five chapters and thirty chapters, including molecular biology basic operation, DNA related experiments, RNA related experiments, gene expression and appendices, introducing the basic principles, experimental procedures and precautions of widely used modern molecular biology experimental techniques. The principle is introduced first, followed by the detailed experimental process, and finally the thinking questions are raised to consolidate the learned content. In the process of introducing the principles and procedures, some practical key tips, reagent effects and possible phenomena are interspersed. The procedures listed in this book are all those used in the author's laboratory, practiced in teaching, and feasible, in line with domestic conditions.