iQue ® Apoptosis Kits product information
The iQue Apoptosis Kits are members of the iQue product line, extensively tested for live cell analysis applications. These screening kits are validated as complete screening assays and are optimal for use in high content flow screening applications. The kits provide a multi-parameter assay with five different apoptosis endpoints per well, including Caspase 3/7 activation, phosphatidylserine surface expression, cell membrane integrity, and mitochondrial membrane potential.
| Assay Volume | 20 µL |
|---|---|
| Cell Density | ~1 × 106 cells/mL |
| Incubation Temperature | 37 °C |
| Incubation Time | 1 hour |
| Storage Temperature | 2–8 °C |
The kits measure Caspase 3/7 activation, phosphatidylserine surface expression, cell membrane integrity, and mitochondrial membrane potential.
No, the kits offer a streamlined workflow with no wash protocols.
Yes, each reagent can be used individually or in multiplex with other apoptosis family reagents or other iQue reagents.